I'm a Ph.D. student in the Lutzoni lab at Duke University. My research focuses on the ecology and evolution of symbioses. I am particularly interested in how mutualistic interactions are structured at different spatial scales and along landscape gradients.
I address these questions using cyanolichens as a model system and within a robust phylogenetic framework.
I always enjoy engaging in conversations about my research or any related topics. I'm also committed to making all resources from my studies available to anyone interested. Please feel free to contact me if you'd like to chat or have any data requests!
New approach to study phylogenetic specialization in ecological networks
A species that interacts with two partners is often considered more specialized than a species that interacts with five. But what if the two partners of the first species are distantly related, and the five partners of the second species are closely related? How do we characterize specialization then? I am developing an approach that integrates phylogenetic structure and partner availability to detect three different patterns of specialization in ecological networks (Fig 1).
Fig 1. Schematic representation of three possible patterns (a–c) of phylogenetic structure (random, clustered, and overdispersed) of partners (guild A) associated with one species from guild B. The size of the orange circles represents the relative availability of each member of guild A in an interaction matrix. Our approach measures the Phylogenetic Structure of Specialization (PSS) to characterize these different types of specialization.
Eco-evolutionary drivers of cyanolichen network structure
I am sequencing over 3000 cyanolichen specimens collected by the ABMI in Alberta, Canada, to study the spatial structure of the interaction network between lichen-forming fungi from the genus Peltigera and their Nostoc cyanobionts. Preliminary results show that at least 28 species of Peltigera are found in Alberta (Fig 2A). In contrast, only six Nostoc phylogroups are found in most thalli. The distribution of these Nostoc phylogroups seems to be spatially structured (Fig 2 B-G). My goal is to study the network properties at different spatial scales and within a robust macroevolutionary framework for both partners.
Fig 2. Association patterns and spatial distribution of the six most common Nostoc phylogroups associated with Peltigera in Alberta based on 157 thalli from 61 ABMI sampling sites. A) Phylogeny of exemplar species representing the eight sections of the genus Peltigera, including 28 species found in association with the six most common Nostoc phylogroups in Alberta. Colored circles in the columns show the Nostoc phylogroups found in association with each species. B-G) Spatial distribution of Nostoc phylogroups found in Peltigera thalli at 61 ABMI sampling sites (circles). Roman numerals correspond to Nostoc phylogroups. Colored circles represent sites where the Nostoc phylogroups associated with Peltigera were found. The size of the colored circles denotes the number of thalli in which a Nostoc phylogroup was found at a given site. “n” is the total number of thalli in which a Nostoc phylogroup was found.
Phylogenetics and interaction patterns in the Peltigera-Nostoc symbioses
During my undergraduate research, I studied lineages of Peltigera that form trimembered thalli, i.e., a symbiosis between a mycobiont (Peltigera), a cyanobacteria (Nostoc) and a green alga (Coccomyxa). I found that two closely related lineages of Peltigera show contrasting patterns of specialization towards their cyanobacterial partners (Fig 3). Species from section Chloropeltigera can associate with up to seven Nostoc phylogroups, while species from section Peltidea associate only with two. I also found evidence that this difference in association patterns might be driven by the type of photobiont transmission. In Peltidea, it is likely that most of the photobiont transmission is vertical, favoring specialization. In Chloropeltigera, photobiont transmission might be mostly horizontal, favoring acquisition of new partners through time.
Fig 3. Phylogenetic relationships among the three Peltigera sections that include trimembered thalli. Thick branches represent ≥70% bootstrap support. Bars on the right indicate the proportion of Peltigera specimens associating with Nostoc phylogroups, Coccomyxa species, and inhabiting various geographic regions. The distribution ranges of the samples are divided into ten broad geographic regions based on latitudinal, oceanic and orographic conditions: Alaska (ALA), Alberta (ALB), Arctic (ARC), Central Europe (CE), Eastern North America (ENA), Mid-Western North America (MWNA), Northern Europe (NE), Pacific North West (PNW), Russia (RUS), and South East Asia (SEA). From Pardo-De la Hoz et al. (2018).
Stone, D. F., McCune, B., Pardo-De la Hoz, C. J., Magain, N., and Miadlikowska, J. (2021). Sinuicella denisonii, a new genus and species in the Peltigeraceae from western North America. The Lichenologist, 53, 185–192. PDF
Miadlikowska, J., Magain, N., Buck, W. R., Vargas Castillo, R., Barlow, G. T., Pardo-De la Hoz, C. J., LaGreca, S., and Lutzoni, F. (2020). Peltigera hydrophila (Lecanoromycetes, Ascomycota), a new semi-aquatic cyanolichen species from Chile. Plant and Fungal Systematics, 65 (1), 210-218. PDF
Miadlikowska, J., Magain, N., Pardo-De la Hoz, C.J., Niu, D., Goward, T., Sérusiaux, E. and Lutzoni, F., 2018. Species in section Peltidea (aphthosa group) of the genus Peltigera remain cryptic after molecular phylogenetic revision. Plant and Fungal Systematics, 63(2), pp.45-64. PDF
Pardo‐De la Hoz, C.J., Magain, N., Lutzoni, F., Goward, T., Restrepo, S. and Miadlikowska, J., 2018. contrasting symbiotic patterns in two closely related lineages of trimembered lichens of the genus Peltigera. Frontiers in Microbiology, 9, p.2770. PDF
Rojas, P., Pardo-De la Hoz, C.J., Calderón, C., Vargas, N., Cabrera, L.A., Restrepo, S. and Jiménez, P., 2018. First Report of Colletotrichum kahawae subsp. ciggaro Causing Anthracnose Disease on Tree Tomato in Cundinamarca, Colombia. Plant Disease, 102(10), pp.2031-2031. PDF
Cabrera, L., Rojas, P., Rojas, S., Pardo‐De la Hoz, C.J., Mideros, M.F., Danies, G., Lopez‐Kleine, L., Jiménez, P. and Restrepo, S., 2018. Most Colletotrichum species associated with tree tomato (Solanum betaceum) and mango (Mangifera indica) crops are not host‐specific. Plant Pathology, 67(5), pp.1022-1030. PDF
Vargas, N., Pardo-De la Hoz, C.J., Danies, G., Franco-Molano, A.E., Jiménez, P., Restrepo, S. and Grajales, A., 2017. Defining the phylogenetic position of Amanita species from Andean Colombia. Mycologia, 109(2), pp.261-276. PDF
Pardo‐De la Hoz, C.J., Calderón, C., Rincón, A.M., Cárdenas, M., Danies, G., López‐Kleine, L., Restrepo, S. and Jiménez, P., 2016. Species from the Colletotrichum acutatum, Colletotrichum boninense and Colletotrichum gloeosporioides species complexes associated with tree tomato and mango crops in Colombia. Plant Pathology, 65(2), pp.227-237. PDF
Department of Biology
130 Science Dr.
Durham, NC, USA